Journal: bioRxiv

Article Title: Neuronal expression of E2F4DN restores adult neurogenesis in homozygous 5xFAD mice via TrkB signaling

doi: 10.1101/2024.12.17.628978

Figure Lengend Snippet: A Gene expression of Bdnf and Ntf5 in cortical cultures from E17 mouse embryos used to generate the conditioned media. Rpl27 expression was used for normalization. B Percentage of Ki67 positive NSCs grown with GFP-or E2F4DN-conditioned medium in the presence or absence of the TrkB blocker TrkB-Fc (left panel). Right panel: scheme of the methodology (right upper panel) and representative immunofluorescence images (right bottom panel). C Percentage of O4 positive cells after NSCs differentiation in GFP-or E2F4DN-conditioned medium in the presence or absence of either TrkB-Fc or the p75 NTR antagonist THX-B (upper panel). Representative images are shown (bottom panel). DAPI was used to counterstain DNA. Scale bars: 20 μm (B); 50 μm (C).

Article Snippet: For BDNF treatment, h5xFAD cultures were treated with 10 ng/ml recombinant BDNF (ThermoFisher Scientific) in self-renewal and proliferation assays, or 50 ng/ml BDNF in the differentiation assay, as previously described by [ ].

Techniques: Expressing, Immunofluorescence

Journal: bioRxiv

Article Title: Neuronal expression of E2F4DN restores adult neurogenesis in homozygous 5xFAD mice via TrkB signaling

doi: 10.1101/2024.12.17.628978

Figure Lengend Snippet: A Gene expression of Bdnf and Ntf5 in the cerebral cortex of h5xFAD animals treated with AAV.GFP or AAV.E2F4DN. B Gene expression of Ntrk2 and Ngfr in WT and h5xFAD NSCs. C Gene expression of Ntrk2 and Ngfr in h5xFAD NSCs grown with GFP-or E2F4DN-conditioned medium. D Gene expression of Ntrk2 and Ngfr in the SVZ of h5xFAD animals treated with AAV.GFP or AAV.E2F4DN. Rpl27 was used to normalize data.

Article Snippet: For BDNF treatment, h5xFAD cultures were treated with 10 ng/ml recombinant BDNF (ThermoFisher Scientific) in self-renewal and proliferation assays, or 50 ng/ml BDNF in the differentiation assay, as previously described by [ ].

Techniques: Expressing

Journal: Cell reports

Article Title: Pten regulates endocytic trafficking of cell adhesion and Wnt signaling molecules to pattern the retina

doi: 10.1016/j.celrep.2024.114005

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Amacrine cell media was supplemented immediately before plating with: BDNF 50 ng/ml (Peprotech, 450–02), CNTF 10 ng/ml (Peprotech, 450–13), TGF-β1 5 ng/ml (Peprotech, 100–21) & TGF-β2 5 ng/ml (Peprotech, 100–35B), Forskolin (1X) (Sigma, F6886).

Techniques: Control, Affinity Purification, Recombinant, Bicinchoninic Acid Protein Assay, Western Blot, Electron Microscopy, RNAscope, Multiplex Assay, Mass Spectrometry, Mutagenesis, Software, Microscopy